PROJECT #14188 RESEARCH FOR Î’-GALACTOSIDASE
FOLDING PERFORMANCE PROFILE

PROJECT SUMMARY

Halorubrum lacusprofundi is a microorganism found in the extremely cold and hypersaline Deep Lake, Antarctica. This microorganism has a polyextremophilic β-galactosidase that works to break down carbohydrates. Recent work from the DasSarma group identified six key amino acid residues in β-galactosidase which, when mutated with amino acids conserved in homologs from mesophilic haloarchaea, induced significant changes to the catalytic efficiency of the enzyme and its temperature dependence (1). 

We are running molecular simulations of β-galactosidase and its mutated variants to explore how these subtle sequence changes affect the behavior of this enzyme at various temperatures. We will analyze the trajectory data to formulate explanations for the mechanisms that cause differences in catalytic efficiencies. 

In the future, a better understanding of how evolved mutations help optimize enzyme efficiency may lead to improved methods for computational enzyme design.

 

References

  1. Laye, V. J., Karan, R., Kim, J.-M., Pecher, W. T., DasSarma, P., & DasSarma, S. (2017). Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic β-galactosidase. Proceedings of the National Academy of Sciences of the United States of America, 114(47), 12530–12535. http://doi.org/10.1073/pnas.1711542114

 

PROJECT INFO

Manager(s): Prof. Vincent Voelz

Institution: Temple University

PROJECT WORK UNIT SUMMARY

Atoms: 127,200

Core: GRO_A7

Status: Public

PROJECT FOLDING PPD AVERAGES BY GPU

PPDDB data as of Wednesday, 28 July 2021 15:34:05

Rank
Project
Model Name
Folding@Home Identifier
Make
Brand
GPU
Model
PPD
Average
Points WU
Average
WUs Day
Average
WU Time
Average

PROJECT FOLDING PPD AVERAGES BY CPU BETA

PPDDB data as of Wednesday, 28 July 2021 15:34:05

Rank
Project
CPU Model Logical
Processors (LP)
PPD-PLP
AVG PPD per 1 LP
ALL LP-PPD
(Estimated)
Make
1 CELERON(R) CPU N3350 @ 1.10GHZ 2 6,045,634 12,091,268 Intel
2 CELERON(R) CPU G550 @ 2.60GHZ 2 3,195,439 6,390,878 Intel
3 A6-3620 APU RADEON(TM) HD GRAPHICS 4 334,312 1,337,248 AMD
4 RYZEN 3 2200G 4 229,288 917,152 AMD
5 EPYC 7302 16-CORE 64 12,908 826,112 AMD
6 RYZEN 9 3950X 16-CORE 32 23,304 745,728 AMD
7 RYZEN 9 5950X 16-CORE 32 14,272 456,704 AMD
8 RYZEN 9 5900X 12-CORE 24 15,325 367,800 AMD
9 EPYC 7302P 16-CORE 32 9,983 319,456 AMD
10 XEON PLATINUM 8124M CPU @ 3.00GHZ 36 8,762 315,432 Intel
11 RYZEN 9 3900XT 12-CORE 24 13,060 313,440 AMD
12 RYZEN THREADRIPPER 2970WX 24-CORE 48 6,527 313,296 AMD
13 RYZEN THREADRIPPER 2950X 16-CORE 32 9,749 311,968 AMD
14 RYZEN 9 3900X 12-CORE 24 12,589 302,136 AMD
15 RYZEN 9 3900 12-CORE 24 11,871 284,904 AMD
16 RYZEN 7 5800X 8-CORE 16 15,555 248,880 AMD
17 CORE I9-9900K CPU @ 3.60GHZ 16 14,561 232,976 Intel
18 RYZEN 7 3700X 8-CORE 16 11,853 189,648 AMD
19 CORE I7-1065G7 CPU @ 1.30GHZ 8 21,664 173,312 Intel
20 XEON CPU E5-2667 V2 @ 3.30GHZ 32 5,165 165,280 Intel
21 XEON CPU E5-2680 V3 @ 2.50GHZ 24 6,453 154,872 Intel
22 RYZEN 7 3800X 8-CORE 16 8,908 142,528 AMD
23 RYZEN 5 PRO 4650G 12 11,614 139,368 AMD
24 CORE I7-10700K CPU @ 3.80GHZ 16 8,610 137,760 Intel
25 RYZEN 5 5600X 6-CORE 12 11,414 136,968 AMD
26 RYZEN 5 3600 6-CORE 12 10,683 128,196 AMD
27 CORE I7-9700K CPU @ 3.60GHZ 8 15,356 122,848 Intel
28 CORE I7-10700F CPU @ 2.90GHZ 16 7,295 116,720 Intel
29 XEON CPU E5-2660 0 @ 2.20GHZ 32 3,637 116,384 Intel
30 RYZEN 7 2700X EIGHT-CORE 16 6,389 102,224 AMD
31 CORE I7-9700 CPU @ 3.00GHZ 8 12,640 101,120 Intel
32 CORE I5-10400F CPU @ 2.90GHZ 12 6,817 81,804 Intel
33 RYZEN 3 3100 4-CORE 8 10,214 81,712 AMD
34 RYZEN 5 2600X SIX-CORE 12 6,642 79,704 AMD
35 RYZEN 5 3500 6-CORE 6 13,167 79,002 AMD
36 RYZEN 7 4800H 16 4,887 78,192 AMD
37 XEON CPU E5-2690 V2 @ 3.00GHZ 20 3,788 75,760 Intel
38 CORE I3-6100 CPU @ 3.70GHZ 4 18,533 74,132 Intel
39 CORE I7-8700K CPU @ 3.70GHZ 12 5,997 71,964 Intel
40 RYZEN 5 1600 SIX-CORE 12 5,360 64,320 AMD
41 RYZEN 7 1700 EIGHT-CORE 16 4,016 64,256 AMD
42 XEON CPU E5-2620 V2 @ 2.10GHZ 24 2,659 63,816 Intel
43 CORE I7-7820HQ CPU @ 2.90GHZ 8 7,729 61,832 Intel
44 CORE I7-6700K CPU @ 4.00GHZ 8 7,691 61,528 Intel
45 CORE I5-9600K CPU @ 3.70GHZ 6 9,762 58,572 Intel
46 CORE I7-3840QM CPU @ 2.80GHZ 8 7,209 57,672 Intel
47 CORE I5-9400F CPU @ 2.90GHZ 6 9,191 55,146 Intel
48 CORE I7-8550U CPU @ 1.80GHZ 8 6,433 51,464 Intel
49 RYZEN 5 2600 SIX-CORE 12 4,262 51,144 AMD
50 CORE I7-8705G CPU @ 3.10GHZ 8 6,353 50,824 Intel
51 CORE I7-4790K CPU @ 4.00GHZ 8 5,606 44,848 Intel
52 11TH GEN CORE I5-1135G7 @ 2.40GHZ 8 5,343 42,744 Intel
53 XEON CPU E5-2690 V3 @ 2.60GHZ 12 3,479 41,748 Intel
54 CORE I5-6300U CPU @ 2.40GHZ 4 10,370 41,480 Intel
55 RYZEN 3 3200G 4 9,875 39,500 AMD
56 CORE I7-4770 CPU @ 3.40GHZ 8 4,892 39,136 Intel
57 XEON CPU E5-2650 0 @ 2.00GHZ 16 2,386 38,176 Intel
58 CORE I3-4160 CPU @ 3.60GHZ 4 9,045 36,180 Intel
59 CORE I5-7600 CPU @ 3.50GHZ 4 7,813 31,252 Intel
60 CORE I7-8565U CPU @ 1.80GHZ 8 3,821 30,568 Intel
61 CORE I7-2600K CPU @ 3.40GHZ 8 3,704 29,632 Intel
62 CORE I7-6700 CPU @ 3.40GHZ 8 3,408 27,264 Intel
63 CORE I7-3770 CPU @ 3.40GHZ 8 3,283 26,264 Intel
64 XEON CPU E3-1225 V3 @ 3.20GHZ 4 6,169 24,676 Intel
65 XEON CPU E3-1230 V2 @ 3.30GHZ 8 3,080 24,640 Intel
66 CORE I7-4930K CPU @ 3.40GHZ 12 2,044 24,528 Intel
67 CORE I5-6600K CPU @ 3.50GHZ 4 5,772 23,088 Intel
68 CORE I5-6500 CPU @ 3.20GHZ 4 5,470 21,880 Intel
69 CORE I3-8100 CPU @ 3.60GHZ 4 4,831 19,324 Intel
70 CORE I7-4790T CPU @ 2.70GHZ 8 2,135 17,080 Intel
71 CORE I5-4300U CPU @ 1.90GHZ 4 4,223 16,892 Intel
72 CORE I7-3610QM CPU @ 2.30GHZ 8 1,985 15,880 Intel
73 CORE I3-7100 CPU @ 3.90GHZ 4 3,941 15,764 Intel
74 CORE I5-6260U CPU @ 1.80GHZ 4 3,734 14,936 Intel
75 CORE I5-4570 CPU @ 3.20GHZ 4 2,974 11,896 Intel
76 PHENOM II X4 965 4 2,825 11,300 AMD
77 RYZEN 5 3500U 8 1,321 10,568 AMD
78 PHENOM II X6 1090T 6 1,749 10,494 AMD
79 CORE I3-6006U CPU @ 2.00GHZ 4 1,985 7,940 Intel
80 RYZEN 5 2500U 8 928 7,424 AMD