PROJECT #14311 RESEARCH FOR MICROORGANISM
FOLDING PERFORMANCE PROFILE

PROJECT SUMMARY

Halorubrum lacusprofundi is a microorganism found in the extremely cold and hypersaline Deep Lake, Antarctica.

This microorganism has a polyextremophilic β-galactosidase that works to break down carbohydrates.

Recent work from the DasSarma group identified six key amino acid residues in β-galactosidase which, when mutated with amino acids conserved in homologs from mesophilic haloarchaea, induced significant changes to the catalytic efficiency of the enzyme and its temperature dependence (1).

We are running molecular simulations of β-galactosidase and its mutated variants to explore how these subtle sequence changes affect the behavior of this enzyme at various temperatures.

We will analyze the trajectory data to formulate explanations for the mechanisms that cause differences in catalytic efficiencies.

In the future, a better understanding of how evolved mutations help optimize enzyme efficiency may lead to improved methods for computational enzyme design. References

  1. Laye, V.

    J., Karan, R., Kim, J.-M., Pecher, W.

    T., DasSarma, P., & DasSarma, S.

    (2017).

    Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic β-galactosidase.

    Proceedings of the National Academy of Sciences of the United States of America, 114(47), 12530–12535.

    http://doi.org/10.1073/pnas.1711542114

.

PROJECT INFO

Manager(s): Prof. Vincent Voelz

Institution: Temple University

PROJECT WORK UNIT SUMMARY

Atoms: 127,200

Core: GRO_A7

Status: Public

PROJECT FOLDING PPD AVERAGES BY GPU

PPDDB data as of Thursday, 29 September 2022 00:15:54

Rank
Project
Model Name
Folding@Home Identifier
Make
Brand
GPU
Model
PPD
Average
Points WU
Average
WUs Day
Average
WU Time
Average

PROJECT FOLDING PPD AVERAGES BY CPU BETA

PPDDB data as of Thursday, 29 September 2022 00:15:54

Rank
Project
CPU Model Logical
Processors (LP)
PPD-PLP
AVG PPD per 1 LP
ALL LP-PPD
(Estimated)
Make
1 RYZEN 9 3950X 16-CORE 32 29,526 944,832 AMD
2 RYZEN 9 5950X 16-CORE 32 12,797 409,504 AMD
3 XEON CPU E5-2690 V4 @ 2.60GHZ 28 5,720 160,160 Intel
4 RYZEN 5 3600 6-CORE 12 10,284 123,408 AMD
5 RYZEN 5 5600X 6-CORE 12 9,479 113,748 AMD
6 RYZEN 5 2600X SIX-CORE 12 9,251 111,012 AMD
7 RYZEN 3 3200G 4 11,681 46,724 AMD
8 CORE I5-7600 CPU @ 3.50GHZ 4 10,881 43,524 Intel
9 CORE I7-7700 CPU @ 3.60GHZ 8 5,119 40,952 Intel
10 CORE I5-7400 CPU @ 3.00GHZ 4 9,633 38,532 Intel
11 CORE I5-6600K CPU @ 3.50GHZ 4 7,187 28,748 Intel
12 CORE I5-4590 CPU @ 3.30GHZ 4 6,982 27,928 Intel
13 XEON CPU @ 2.30GHZ 1 1,191 1,191 Intel