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PROJECT #16925 RESEARCH FOR Î’-GALACTOSIDASE
FOLDING PERFORMANCE PROFILE

PROJECT SUMMARY

Halorubrum lacusprofundi is a microorganism found in the extremely cold and hypersaline Deep Lake, Antarctica. This microorganism has a polyextremophilic β-galactosidase that works to break down carbohydrates. Recent work from the DasSarma group identified six key amino acid residues in β-galactosidase which, when mutated with amino acids conserved in homologs from mesophilic haloarchaea, induced significant changes to the catalytic efficiency of the enzyme and its temperature dependence (1). 

We are running molecular simulations of β-galactosidase and its mutated variants to explore how these subtle sequence changes affect the behavior of this enzyme at various temperatures. We will analyze the trajectory data to formulate explanations for the mechanisms that cause differences in catalytic efficiencies. 

In the future, a better understanding of how evolved mutations help optimize enzyme efficiency may lead to improved methods for computational enzyme design.

 

References

  1. Laye, V. J., Karan, R., Kim, J.-M., Pecher, W. T., DasSarma, P., & DasSarma, S. (2017). Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic β-galactosidase. Proceedings of the National Academy of Sciences of the United States of America, 114(47), 12530–12535. http://doi.org/10.1073/pnas.1711542114

 

PROJECT INFO

Manager(s): Prof. Vincent Voelz

Institution: Temple University

PROJECT WORK UNIT SUMMARY

Atoms: 127,200

Core: GRO_A7

Status: Public

PROJECT FOLDING PPD AVERAGES BY GPU

PPDDB data as of Friday, 03 December 2021 04:36:10

Rank
Project 		Model Name
Folding@Home Identifier 		Make
Brand 		GPU
Model 		PPD
Average 		Points WU
Average 		WUs Day
Average 		WU Time
Average
1 HD Graphics 4000
[HD Graphics 4000]		 Intel 341 1,360 0.25 96 hrs 43 mins

PROJECT FOLDING PPD AVERAGES BY CPU BETA

PPDDB data as of Friday, 03 December 2021 04:36:10

Rank
Project 		CPU Model Logical
Processors (LP) 		PPD-PLP
AVG PPD per 1 LP 		ALL LP-PPD
(Estimated) 		Make
1 RYZEN THREADRIPPER 3970X 32-CORE 64 16,740 1,071,360 AMD
2 RYZEN THREADRIPPER 3960X 24-CORE 48 15,812 758,976 AMD
3 RYZEN 9 5950X 16-CORE 32 18,042 577,344 AMD
4 RYZEN 9 3950X 16-CORE 32 14,585 466,720 AMD
5 RYZEN 9 5900X 12-CORE 24 16,636 399,264 AMD
6 RYZEN 9 3900X 12-CORE 24 14,438 346,512 AMD
7 RYZEN 9 3900XT 12-CORE 24 13,784 330,816 AMD
8 CORE I9-10980XE CPU @ 3.00GHZ 36 8,618 310,248 Intel
9 XEON CPU E5-2686 V4 @ 2.30GHZ 36 7,770 279,720 Intel
10 CORE I9-10900K CPU @ 3.70GHZ 20 13,627 272,540 Intel
11 RYZEN THREADRIPPER 2970WX 24-CORE 48 5,448 261,504 AMD
12 RYZEN THREADRIPPER 2950X 16-CORE 32 7,818 250,176 AMD
13 CORE I9-10900X CPU @ 3.70GHZ 20 9,560 191,200 Intel
14 RYZEN 7 5800X 8-CORE 16 11,839 189,424 AMD
15 CORE I9-7920X CPU @ 2.90GHZ 24 7,827 187,848 Intel
16 RYZEN THREADRIPPER 1920X 12-CORE 24 7,790 186,960 AMD
17 CORE I9-9900K CPU @ 3.60GHZ 16 11,648 186,368 Intel
18 RYZEN 7 3800X 8-CORE 16 11,347 181,552 AMD
19 CORE I9-9900KF CPU @ 3.60GHZ 16 10,557 168,912 Intel
20 CORE I9-7940X CPU @ 3.10GHZ 28 5,961 166,908 Intel
21 XEON W-2245 CPU @ 3.90GHZ 16 10,364 165,824 Intel
22 RYZEN 7 4800H 16 10,163 162,608 AMD
23 RYZEN 7 3700X 8-CORE 16 9,859 157,744 AMD
24 RYZEN 5 3600X 6-CORE 12 12,809 153,708 AMD
25 RYZEN 5 3600 6-CORE 12 12,008 144,096 AMD
26 RYZEN 5 3600XT 6-CORE 12 11,067 132,804 AMD
27 RYZEN 7 2700X EIGHT-CORE 16 8,275 132,400 AMD
28 XEON CPU E5-2660 0 @ 2.20GHZ 32 3,659 117,088 Intel
29 CORE I7-8700K CPU @ 3.70GHZ 12 9,081 108,972 Intel
30 XEON CPU E5-2670 0 @ 2.60GHZ 32 3,283 105,056 Intel
31 CORE I7-8700 CPU @ 3.20GHZ 12 8,353 100,236 Intel
32 CORE I7-9700 CPU @ 3.00GHZ 8 11,966 95,728 Intel
33 CORE I7-9700K CPU @ 3.60GHZ 8 11,109 88,872 Intel
34 CORE I5-10400F CPU @ 2.90GHZ 12 7,145 85,740 Intel
35 RYZEN 3 3100 4-CORE 8 10,637 85,096 AMD
36 RYZEN 5 3500 6-CORE 6 14,155 84,930 AMD
37 RYZEN 7 1800X EIGHT-CORE 16 5,173 82,768 AMD
38 XEON CPU X5570 @ 2.93GHZ 16 4,997 79,952 Intel
39 XEON CPU E5-2690 V2 @ 3.00GHZ 20 3,989 79,780 Intel
40 XEON CPU E5-2630 0 @ 2.30GHZ 24 3,253 78,072 Intel
41 CORE I7-10710U CPU @ 1.10GHZ 12 6,438 77,256 Intel
42 CORE I5-6600 CPU @ 3.30GHZ 4 18,673 74,692 Intel
43 RYZEN 5 2600X SIX-CORE 12 6,109 73,308 AMD
44 RYZEN 7 1700 EIGHT-CORE 16 4,373 69,968 AMD
45 RYZEN 7 1700X EIGHT-CORE 16 4,234 67,744 AMD
46 CORE2 DUO CPU T7700 @ 2.40GHZ 12 5,372 64,464 Intel
47 CORE I7-4750HQ CPU @ 2.00GHZ 8 7,563 60,504 Intel
48 RYZEN 5 4600HS 12 4,993 59,916 AMD
49 XEON SILVER 4116 CPU @ 2.10GHZ 24 2,461 59,064 Intel
50 CORE I7-4980HQ CPU @ 2.80GHZ 8 7,251 58,008 Intel
51 EPYC (IBPB) 12 4,795 57,540 AMD
52 XEON CPU E5-2620 V2 @ 2.10GHZ 24 2,332 55,968 Intel
53 CORE I7-7700K CPU @ 4.20GHZ 8 6,931 55,448 Intel
54 RYZEN 5 1600 SIX-CORE 12 4,618 55,416 AMD
55 RYZEN 5 2600 SIX-CORE 12 4,566 54,792 AMD
56 RYZEN 5 1600X SIX-CORE 12 4,547 54,564 AMD
57 CORE I3-10100 CPU @ 3.60GHZ 8 6,763 54,104 Intel
58 CORE I5-8400 CPU @ 2.80GHZ 6 8,568 51,408 Intel
59 XEON CPU E3-1270 V5 @ 3.60GHZ 8 6,264 50,112 Intel
60 CORE I7-6820HQ CPU @ 2.70GHZ 8 6,132 49,056 Intel
61 CORE I7-4790K CPU @ 4.00GHZ 8 6,043 48,344 Intel
62 CORE I9-9880H CPU @ 2.30GHZ 16 3,008 48,128 Intel
63 RYZEN THREADRIPPER 1900X 8-CORE 16 2,971 47,536 AMD
64 CORE I5-9400 CPU @ 2.90GHZ 6 7,872 47,232 Intel
65 CORE I7-9700F CPU @ 3.00GHZ 8 5,513 44,104 Intel
66 CORE I7-8705G CPU @ 3.10GHZ 8 5,034 40,272 Intel
67 RYZEN 7 2700 EIGHT-CORE 16 2,507 40,112 AMD
68 CORE I7-4790 CPU @ 3.60GHZ 8 4,700 37,600 Intel
69 CORE I5-7600 CPU @ 3.50GHZ 4 9,256 37,024 Intel
70 CORE I7-6700K CPU @ 4.00GHZ 8 4,628 37,024 Intel
71 XEON CPU E3-1535M V5 @ 2.90GHZ 8 4,570 36,560 Intel
72 CORE I3-8100 CPU @ 3.60GHZ 4 8,970 35,880 Intel
73 CORE I5-6500 CPU @ 3.20GHZ 4 8,285 33,140 Intel
74 CORE I5-3570K CPU @ 3.40GHZ 4 7,855 31,420 Intel
75 CORE I7-2600K CPU @ 3.40GHZ 8 3,655 29,240 Intel
76 CORE I7-3770 CPU @ 3.40GHZ 8 3,391 27,128 Intel
77 CORE I5-9600KF CPU @ 3.70GHZ 6 4,059 24,354 Intel
78 CORE I5-8265U CPU @ 1.60GHZ 8 2,906 23,248 Intel
79 CORE I7-6700HQ CPU @ 2.60GHZ 8 2,557 20,456 Intel
80 XEON E-2124G CPU @ 3.40GHZ 4 4,819 19,276 Intel
81 PHENOM II X6 1090T 6 3,200 19,200 AMD
82 CORE I5-4570 CPU @ 3.20GHZ 4 4,387 17,548 Intel
83 CORE I3-6100 CPU @ 3.70GHZ 4 4,372 17,488 Intel
84 CORE I5-2500K CPU @ 3.30GHZ 4 4,042 16,168 Intel
85 FX-8350 EIGHT-CORE 8 1,964 15,712 AMD
86 RYZEN 3 2200G 4 3,736 14,944 AMD
87 CORE I5-6200U CPU @ 2.30GHZ 4 3,342 13,368 Intel
88 CORE I5-7300HQ CPU @ 2.50GHZ 4 3,269 13,076 Intel
89 CORE I5-4300U CPU @ 1.90GHZ 4 2,974 11,896 Intel
90 PHENOM II X6 1055T 6 1,682 10,092 AMD
91 CORE I7-3520M CPU @ 2.90GHZ 4 2,502 10,008 Intel
92 CORE I7-1065G7 CPU @ 1.30GHZ 8 1,189 9,512 Intel
93 CORE I5-4460 CPU @ 3.20GHZ 4 2,296 9,184 Intel
94 XEON CPU L5420 @ 2.50GHZ 4 2,029 8,116 Intel
95 RYZEN 5 3500U 8 947 7,576 AMD
96 CORE I3-6006U CPU @ 2.00GHZ 4 1,841 7,364 Intel
97 CORE I5-4200U CPU @ 1.60GHZ 4 1,830 7,320 Intel
98 CORE I5-3320M CPU @ 2.60GHZ 4 1,459 5,836 Intel
99 CORE I5-3317U CPU @ 1.70GHZ 4 1,109 4,436 Intel
100 CORE2 DUO CPU E6750 @ 2.66GHZ 2 1,804 3,608 Intel
101 CORE2 DUO CPU T8100 @ 2.10GHZ 2 1,324 2,648 Intel
102 A8-6410 APU 4 39 156 AMD